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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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R model and IV tail injection metastasis model relative to vector controls [27]. In human breast cancer, high TXNIP levels are associated with longer metastasis-free intervals and better prognosis than those with low TXNIP expression [43,46]. These data implicate TXNIP as a tumor suppressor in a variety of cancers and, for the first time, is now shown to be a tumor suppressor in thyroid cells. Cur
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Vitro cell culture conditions do not adequately recapitulate the tumor microenvironment and contributions of paracrinemediated signaling, underscoring the importance of in vivo studies. In accordance with this potential limitation, Goldberg and colleagues failed to see slowed in vitro growth of melanoma cells transfected with TXNIP though when injected in an orthotopic flank model in nude mice, sl
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Uares () indicate vector control cells, and open diamonds () indicate cells with TXNIP overexpression. In vitro invasion assays were performed on the TXNIP-overexpressing stable HTh74 (C) and T238 (D) cell lines as described in the Methods section. Results from three independent experiments were combined and normalized to the vector control average and graphed with mean plus SEM. *p