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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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Uares () indicate vector control cells, and open diamonds () indicate cells with TXNIP overexpression. In vitro invasion assays were performed on the TXNIP-overexpressing stable HTh74 (C) and T238 (D) cell lines as described in the Methods section. Results from three independent experiments were combined and normalized to the vector control average and graphed with mean plus SEM. *p
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Uares () indicate vector control cells, and open diamonds () indicate cells with TXNIP overexpression. In vitro invasion assays were performed on the TXNIP-overexpressing stable HTh74 (C) and T238 (D) cell lines as described in the Methods section. Results from three independent experiments were combined and normalized to the vector control average and graphed with mean plus SEM. *p
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Alized to vector control levels (average set at 1). **p = 0.001, ***p
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Ated development of high grade and invasive tumors by 4 weeks compared to controlswith wild-type expression, however, controls eventually succumb to tumor development and TXNIP expression in these tumors has been downregulated by other mechanisms [24]. In the orthotopic ATC model, TXNIP overexpression also led to a significant reduction in pulmonary metastatic burden. Inhibition of metastasis con
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates