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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
1
Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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Sitive family history of confirmed cardiovascular diseases considering first degree relatives was completed. Hypertension, when the patient was not taking any medication, was defined as systolic blood pressure 140 mmHg and/or diastolic blood pressure 90 mmHg and diabetes mellitus as two fasting plasma glucose levels of 126 mg/dL or higher. Patient medication was recorded in detail and categorized
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Ean RT, Brown AJ: Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function? Biochem J 1998, 331(1):231?37. 4. Ahuja HS, Tenniswood M, Lockshin R, Zakeri ZF: Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 1994, 72(11?2):523?30. 5. Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Cluste
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Ean RT, Brown AJ: Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function? Biochem J 1998, 331(1):231?37. 4. Ahuja HS, Tenniswood M, Lockshin R, Zakeri ZF: Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 1994, 72(11?2):523?30. 5. Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Cluste
1
Sitive family history of confirmed cardiovascular diseases considering first degree relatives was completed. Hypertension, when the patient was not taking any medication, was defined as systolic blood pressure 140 mmHg and/or diastolic blood pressure 90 mmHg and diabetes mellitus as two fasting plasma glucose levels of 126 mg/dL or higher. Patient medication was recorded in detail and categorized
1
Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
1
Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1