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Nsformation. Cells of N. meningitidis strain H44/76 (B:15: P1.7,16:L3,7) incubated overnight in the presence of 5 CO2 on chocolate base (GC) (Difco) or Mueller-Hinton (MH) (Difco) medium plates were collected in 2 ml of liquid GC or MH medium containing 10 mM MgCl2 and diluted to obtain an optical density at 550 nm (OD550) of 0.1. Two micrograms of DNA was added to the cell suspension, and this w
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