Utes and for measuring intrinsic GTPase activity 2 mM RbgA protein was incubated with 200 mM GTP at 37uC for 30 minutes. We predetermined that under these conditions the values were in the linear range of the assay. The phosphate released was measured using the Malachite Green Phosphate Assay Kit (BioAssaySystems).Quantitative mass spectrometryExperimental samples were prepared for quantitative ma
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