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Used as blank and subtracted from all the active curves to correct background effects. The association, dissociation and affinity constants (ka e kd and KD respectively) were determined by a simultaneous fitting of the kinetic curves with a model of equimolar stoichiometry (1:1) using the BIAevaluation X100 software version 1.0 (GE Healthcare).Generation of mouse monoclonal antibodiesMouse monoclo
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