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Tandard still allowed for independent quantitation of the 15N isotope distribution for each peptide, but simultaneously ensured that each 15N peak bore a corresponding 14N peak pair irrespective of the concentration in the experimental sample. After precipitation, reduction, alkylation and tryptic digestion, peptides were analyzed on an Agilent G1969A ESI-TOF mass spectrometer. 14N:15N peak pairs
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