Phase bacterial cells were fixed in 0.08 (wt/vol) paraformaldehyde and incubated for 1 hour at 37uC. Fixed bacteria were then washed once with PBS, resuspended in Newborn Calf Serum (Sigma) and incubated for 20 min. at 25uC. The cells were then incubated for 1 hour at 4uC in presence of mAbs diluted 1:200 in dilution buffer (PBS, 20 Newborn Calf Serum, 0.1 BSA). Cells were washed in PBS-0.1 BS
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